Thymidylate Synthase (TYMS) Mouse Monoclonal Antibody [Clone ID: AT1S5]

CAT#: AM09355PU-N

Thymidylate Synthase (TYMS) mouse monoclonal antibody, clone AT1S5, Purified

Product Images

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TYMS (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TYMS (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  HeLa cells were stained with monoclonal anti-TYMS antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).

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  Immunofluorescence: HeLa cells were stained with monoclonal anti-TYMS antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).

Specifications

Product Data

• Clone Name: AT1S5
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:1,000).
  Immunofluorescence (1:500 - 1:1,000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant TYMS (1-313 aa) purified from E. coli
• Specificity: The antibody recognizes human TYMS. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens thymidylate synthetase (TYMS)
• Database Link:
  Entrez Gene 7298 Human
• Background: The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS). TYMS is an intracellular enzyme that provides the sole de novo source of thymidylate in activity highest proliferating cells. Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head and neck. TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers.
• Synonyms: TYMS, TSase, OK/SW-cl.29

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism