ornithine aminotransferase (OAT) Mouse Monoclonal Antibody [Clone ID: AT23A2]

CAT#: AM09362PU-S

ornithine aminotransferase (OAT) mouse monoclonal antibody, clone AT23A2, Purified

Product Images

Western blot analysis: Tissue lysates of mouse liver (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of human HeLa cells stained with monoclonal anti-human OAT anitbody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Specifications

Product Data

• Clone Name: AT23A2
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1/250-1/1000).
  Flow Cytometry.
  Immunofluorescence (1/250-1/500).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human OAT (33-439aa) purified from E. coli
• Specificity: The antibody recognizes Human and Mouse OAT. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ornithine aminotransferase (OAT), transcript variant 1
• Database Link:
  Entrez Gene 18242 MouseEntrez Gene 4942 Human
• Background: OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.
• Synonyms: Ornithine aminotransferase mitochondrial

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways