LDHA Mouse Monoclonal Antibody [Clone ID: AT1A4]

CAT#: AM09366PU-N

LDHA mouse monoclonal antibody, clone AT1A4, Purified

Product Images

Western blot analysis: Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

Specifications

Product Data

• Clone Name: AT1A4
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/8000).
  Immunofluorescence (1/500-1/8000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human LDHA (1-332aa) purified from E. coli
• Specificity: The antibody recognizes Human LDHA. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1
• Database Link:
  Entrez Gene 3939 Human
• Background: Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.
• Synonyms: LDH-A, L-lactate dehydrogenase A chain, LDH-M, PIG19, NY-REN-59

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism