ERK2 (MAPK1) Mouse Monoclonal Antibody [Clone ID: AT1A4]

CAT#: AM09368PU-S

ERK2 (MAPK1) mouse monoclonal antibody, clone AT1A4, Purified

Product Images

Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• 

  Western blot analysis: Cell lysates of HepG2 (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• 

  Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).

• 

  Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).

Specifications

Product Data

• Clone Name: AT1A4
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:3,000).
  Immunofluorescence (1:500 - 1:3,000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human MAPK1 (1-360aa) purified from E. coli
• Specificity: The antibody recognizes human MAPK1. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1
• Database Link:
  Entrez Gene 5594 Human
• Background: Mitogen-activated protein kinases (MAPKs) are reported to be critical regulatory factors for the growth andmigration of various cell types including vascular smooth muscle cells (VSMCs). It has also been reported that the activation of the MAP kinase family, extracellular signal regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) via Src activation is important for AII-induced migration of VSMCs. MAPKs are intracellular signal-transduction pathways that have been shown to play a central role in the development of injury following ischemia in the brain and heart. MAPKs regulate gene expression, which is important in cell injury/repair and proliferation/differentiation.
• Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway