NMNAT1 Mouse Monoclonal Antibody [Clone ID: AT4A2]

CAT#: AM09374PU-N

NMNAT1 mouse monoclonal antibody, clone AT4A2, Purified

Product Images

Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NMNAT1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of HepG2 (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NMNAT1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT4A2
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:1000).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human NMNAT1 (1-279aa) purified from E. coli
• Specificity: The antibody recognizes human NMNAT1. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-G affinity chromatography
• Gene Name: Homo sapiens nicotinamide nucleotide adenylyltransferase 1 (NMNAT1), transcript variant 1
• Database Link:
  Entrez Gene 64802 Human
• Background: Nicotinamide mononucleotide adenylyltransferase 1 is an enzyme that in humans is encoded by the NMNAT1 gene. It is an essential enzyme because it catalyzes the final step of NAD+ biosynthesis. There are three isoforms in humans that exhibit tissue- and organelle-specific expression. NMNAT-1 is localized exclusively in the nucleus, whereas NMNAT-2 and NMNAT-3 are found in the Golgi and mitochondria, respectively. NMNAT1 constitute a nuclear NAD+ salvage pathway which regulates the functions of NAD+-dependent enzymes such as the protein deacetylase SIRT1. NMNAT1 protects neurons from neuronal activity induced degeneration.
• Synonyms: NMNAT, NMN adenylyltransferase 1

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism