BCL2 (1-211) Mouse Monoclonal Antibody [Clone ID: AT1B5]

CAT#: AM09378PU-N

BCL2 (1-211) mouse monoclonal antibody, clone AT1B5, Purified

Product Images

Western blot analysis: Cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Bcl-2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and with monoclonal anti-human Bcl-2 antibody (1:500) with Texas Red (Red).

Specifications

Product Data

• Clone Name: AT1B5
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:250 - 1:500).
  Immunofluorescence (1:250 - 1:500).
  Flow cytometry.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human Bcl-2 (1-211aa) purified from E. coli
• Specificity: The antibody recognizes Bcl-2 (B-cell lymphoma protein 2 alpha) at aa 1-211.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens B-cell CLL/lymphoma 2 (BCL2), transcript variant alpha
• Database Link:
  Entrez Gene 12043 MouseEntrez Gene 596 Human
• Background: Bcl-2 (B-cell lymphoma protein 2 alpha) is an anti-apoptotic protein located primarily in the outer mitochondrial membrane. It blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apoptosis. Bcl-2 protein has anti-apoptotic functions through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
• Synonyms: BCL2, Bcl-2 alpha

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Focal adhesion, Neurotrophin signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer