Caveolin 1 (CAV1) (1-104) Mouse Monoclonal Antibody [Clone ID: AT4C1]

CAT#: AM09383PU-S

Caveolin 1 (CAV1) (1-104) mouse monoclonal antibody, clone AT4C1, Purified

Product Images

Rat brain lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAV1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT4C1
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA (starting dilution is 1:500).
  Western blot (1:250 - 1:1000, starting dilution is 1:500).
  Immunohistochemistry / Immunoflourescence.
  Flow cytometry.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human CAV1 (1-104aa) purified from E. coli
• Specificity: The antibody recognizes caveolin-1 at aa 1-104.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens caveolin 1 (CAV1), transcript variant 1
• Database Link:
  Entrez Gene 25404 RatEntrez Gene 857 Human
• Background: The identification of caveolin-1 (CAV1) as the main structural component of caveolae, together with the finding that CAV1 might serve as a molecular organizer for membrane multiprotein complexes involved in cellular traffic, endo-and transcytosis, cell adhesion and signal transduction prompted a new impulse in the research on these intracellular organells. One of the properties of CAV1 was its insolubility in cold non-ionic detergents together with apical markers in epithelial cell. Also, cholesterol is essential for caveolae formation and maintenance, and caveolae structure is highly sensitive to cholesterol depletion or treatment with cholesterol binding drugs. CAV1 tightly and specifically binds free cholesterol and artificial cholesterol containing vesicles.
• Synonyms: CAV1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Focal adhesion, Viral myocarditis