ADK (22-362) Mouse Monoclonal Antibody [Clone ID: AT4F8]

CAT#: AM09394PU-S

ADK (22-362) mouse monoclonal antibody, clone AT4F8, Purified

Product Images

Western blot analysis: The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT4F8
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:1000).
  Immunocytochemistry / Immunoflourescence.
  Flow cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human ADK (22-362aa) purified from E. coli
• Specificity: The antibody recognizes human Adenosine kinase at aa 22-362. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-G affinity chromatography
• Gene Name: Homo sapiens adenosine kinase (ADK), transcript variant 1
• Database Link:
  Entrez Gene 132 Human
• Background: Adenosine kinase (ADK) is the key regulator of adenosine metabolism. Because of the manifold receptor-dependent actions of adenosine, tight regulation of adenosine levels is crucial. The intracellular and extracellular pools of adenosine are in dynamic exchange by equilibrative and concentrative nucleoside transporters, so extracellular concentrations of adenosine are regulated by interplay of theses transporters with intracellular and extracellular enzymes of adenosine metabolism. Thus, the extracellular concentration of adenosine is enhanced by inhibition of equilibrative nucleoside transporters such as S-(4-nitrobenzyl)-6-thioinosine and cannabidiol and, stimulation of extracellular ATP breakdown and inhibition of intracellular adenosine removal.
• Synonyms: ADK, AK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism