elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: AT5E10]

CAT#: AM09396PU-N

elF2 alpha (EIF2S1) mouse monoclonal antibody, clone AT5E10, Purified

Product Images

The extracts of HeLa (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: The extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT5E10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western blot (1:1,000).
  Immunohistochemistry on Paraffin Sections (10 µg/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant human EIF2S1 (1-315aa) purified from E. coli
• Specificity: The antibody recognizes human EIF2S1. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens eukaryotic translation initiation factor 2 subunit alpha (EIF2S1)
• Database Link:
  Entrez Gene 1965 Human
• Background: Eukaryotic translation initiation factor 2, subunit 1 alpha (EIF2S1) is plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. The phosphorylation of alpha-subunit of EIF2S1 effectively prevents formation of the EIF2S1/GTP/Met-tRNAi complex and inhibits global protein synthesis.
• Synonyms: EIF2-A, eIF-2A, eIF-2-alpha, eIF-2alpha, eIF2 alpha