Pyruvate Kinase (PKLR) Mouse Monoclonal Antibody [Clone ID: AT1E3]

CAT#: AM09397PU-S

Pyruvate Kinase (PKLR) mouse monoclonal antibody, clone AT1E3, Purified

Product Images

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• 

  Western blot analysis: The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKLR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT1E3
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1:500).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human PKLR (47-574aa) purified from E. coli
• Specificity: The antibody recognizes human PKLR. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens pyruvate kinase, liver and RBC (PKLR), transcript variant 1
• Database Link:
  Entrez Gene 5313 Human
• Background: PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
• Synonyms: PK1, PKL, Pyruvate kinase R/L, Pyruvate kinase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Purine metabolism, Pyruvate metabolism, Type II diabetes mellitus