Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: CK209]

CAT#: AM10025FC-S

Cytokeratin 18 (KRT18) mouse monoclonal antibody, clone CK209, FITC

Specifications

Product Data

• Clone Name: CK209
• Applications: FC, IF, IHC
• Recommended Dilution: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears).
  For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at RT.
  Antigen retriever like Citrate enhances the staining of FFPE tissue sections (Boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT).
  Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or Overnight at 4ºC.
  Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000).
  Recommended Positive Control: Human skin, breast, lung ca.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human breast cancer PMC 42 cells
• Specificity: This anitbody recognises Cytokeratin 18 (CK 18).
  Varieties of simple epithelia including glandular but not stratified squamous epithelia are detected by this antibody.
  Cellular Localization: Cytoplasmic
• Formulation: PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative
  Label: FITC
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 3875 Human
• Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.GI tract contain both CK 8 and CK18 but not CK 14. Cytokeratin is expressed in GI and respiratory tract, mesothelial cells, endocrine and exocrine tissues.
• Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18