basic Cytokeratin Mouse Monoclonal Antibody [Clone ID: AE3]

CAT#: AM10030FC-S

basic Cytokeratin mouse monoclonal antibody, clone AE3, FITC, Purified

Specifications

Product Data

• Clone Name: AE3
• Applications: FC, IF
• Recommended Dilution: Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
  Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
• Reactivities: Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human epidermal keratin.
• Specificity: This antibody reacts with CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58) CK6(56) and CK8 (52.5).
  Cellular Localization:Cytoplasmic.
• Formulation: PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative
  Label: FITC
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.2 mg/ml
• Purification: Protein A Chromatography
• Conjugation: FITC
• Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).