p53 (TP53) Mouse Monoclonal Antibody [Clone ID: BP53-12]

CAT#: AM10034FC-N

p53 (TP53) mouse monoclonal antibody, clone BP53-12, FITC


USD 405.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name BP53-12
Applications FC, IF, IHC
Recommended Dilution Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears)
For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins).
Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000).
Recommended Positive Control: Human colon Cancer.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Recombinant Human wild type p53 protein.
Specificity This antibody is specific for p53. Other species not tested.
Cellular Localization: Nuclear.
Formulation PBS, pH 7.4
Label: FITC
State: Liquid purified Ig fraction
Stabilizer: 0.05% Sodium Azide
Preservative: 0.05% Sodium Azide
Concentration lot specific
Conjugation FITC
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Stability Shelf life: One year from despatch.
Background P53, also known as protein 53 (TP53), is a transcription factor that regulates the cell cycle and hence functions as a tumor suppressor in a variety of tissue types and is involved in regulating cell growth, replication and apoptosis. It binds mdm2, SV40 T antigen and human papilloma virus E6 protein, p53 senses DNA damage and facilitate response. The human gene that encodes for p53 is TP53. The gene is named TP53 after the protein it codes for (TP53 is another name for p53). The gene is located on the human chromosome 17 (17p13.1. Mutation involving p53 is found in a variety of malignant tumors, including breast, ovarian, bladder, colon, lung and melanoma.
Synonyms Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Note Clone name P53215 is the same as BP53-12
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.