LOC104968698 Mouse Monoclonal Antibody [Clone ID: S100217]

CAT#: AM10036PU-S

LOC104968698 mouse monoclonal antibody, clone S100217, Purified


USD 280.00

2 Weeks*

Size
    • 500 ul

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Specifications

Product Data
Clone Name S100217
Applications FC, IF, IHC
Recommended Dilution Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears)
For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins).
Immunofluorescence.
Flow Cytometry.

Recommended Positive Control: Melanoma or schwannoma.
Reactivities Bovine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified bovine S100 protein.
Specificity This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.
Cellular Localization: Cytoplasmic.
Formulation PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
State: Purified
State: Liquid purified Ig fraction.
Concentration 0.2 mg/ml
Purification Protein A Chromatography.
Background S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH. Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.
Synonyms S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain, S-100 protein beta chain, Astrocyte Marker
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.