CD175s Mouse Monoclonal Antibody [Clone ID: STN219]

CAT#: AM10038FC-N

CD175s mouse monoclonal antibody, clone STN219, FITC

Specifications

Product Data

• Clone Name: STN219
• Applications: FC, IF, IHC
• Recommended Dilution: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears).
  For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
  Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
  Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000).
  Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Ovine submaxillary mucin (OSM).
• Specificity: The specificity of this antibody is similar to B 72.3 antibody.
  Cellular Localization: Cytoplasmic, apical membrane, luminal.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified Ig fraction.
  Stabilizer: 1% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Conjugation: FITC
• Background: Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma.
  The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
• Synonyms: Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn