Bcl-2 Mouse Monoclonal Antibody [Clone ID: 124]

CAT#: AM10050SU-N

Bcl-2 mouse monoclonal antibody, clone 124, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human reactive lymph node stained with Bcl-2 antibody Cat.-No. AM10050SU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic and membrane staining of mantle B-cells cells and perifollicular T-cells and no staining of reactive B-cells in the germinal centers.

Specifications

Product Data

• Clone Name: 124
• Applications: IHC, WB
• Recommended Dilution: Western blot.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Tonsil or lymph node.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide corresponduing to amino acids 41-54 of Human Bcl-2 protein.
• Specificity: This antibody is specific to bcl-2 oncoprotein encoded by a gene involved in the 14; 18 chromosomal translocation.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Background: Bcl-2 protein family members function either to promote or inhibit programmed cell death. Some of these proteins, such as Bax and Bak, function to promote apoptosis, whereas others like Bcl-2 and Bcl-XL inhibit apoptosis. Bcl-2, typically an inhibitor of apoptosis, has also been demonstrated to have pro-apoptotic activity. The Bcl-2 protein is a suppressor of apoptosis that homodimerizes with itself and forms heterodimers with Bax.
  Expression of Bcl-2α oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2α protein, whereas the normal or hyperplastic germinal centers are negative.
• Synonyms: BCL2, Bcl-2 alpha