Caldesmon (CALD1) Rabbit Monoclonal Antibody [Clone ID: EP19]

CAT#: AM10053SU-N

Caldesmon (CALD1) rabbit monoclonal antibody, clone EP19, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human uterus sections stained with Caldesmon antibody Cat.-No. AM10053SU-N using peroxidase conjugate and AEC chromogen. Note cytoplasmic staining of smooth muscle cells.

Specifications

Product Data

• Clone Name: EP19
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry.
  Immunocytochemistry.
  Western blot: A band at approximately 120-150 /70-80 is detected.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100.
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Uterus, colon carcinoma.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser789 of Human Caldesmon.
• Specificity: This antibody Recognizes Human Caldesmon.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens B-cell CLL/lymphoma 6 (BCL6), transcript variant 2
• Database Link:
  Entrez Gene 604 HumanEntrez Gene 800 Human
• Background: Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Two closely related variants of Human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-Caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-Caldesmon (70-80kDa) is found in non-muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
• Synonyms: CALD1, CAD, CDM

Reference Data

• Protein Families: Druggable Genome, Transcription Factors