CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: JC70A]

CAT#: AM10064SU-N

CD31 (PECAM1) mouse monoclonal antibody, clone JC70A, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human skin sections stained with CD31 antibody Cat.-No. AM10064SU-N using peroxidase conjugate and DAB chromogen. Note membranous staining of vascular endothelial cells.

Specifications

Product Data

• Clone Name: JC70A
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Placenta.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Preparation of a spleen from a patient with hairy cell leukemia.
• Specificity: This antibody Recognizes CD31/PECAM1.
  JC/70A reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In Cryostat Sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells.1 It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils.
  Cellular Localization: Cell membrane.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD163 molecule (CD163), transcript variant 1
• Database Link:
  Entrez Gene 9332 HumanEntrez Gene 5175 Human
• Background: CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.
  CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
  
  CD31 is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney as well as in skin biopsies from conditions such as sarcoidosis and lepromatous leprosy.
• Synonyms: PECAM-1, EndoCAM, GPIIA'

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane