CD45 (PTPRC) (CD45RA) Mouse Monoclonal Antibody [Clone ID: 4KB5]

CAT#: AM10067SU-N

CD45 (PTPRC) (CD45RA) mouse monoclonal antibody, clone 4KB5, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human lymph node stained with CD45RA antibody Cat.-No. AM10067SU-N using peroxidase conjugate and DAB chromogen. Note the membrane staining of B-cells in mantle (strong) and in follicle (weak).

Specifications

Product Data

• Clone Name: 4KB5
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Lymph node or tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hairy cell leukemia cells.
• Specificity: Clone 4KB4 is useful in study on the subpopulation of CD4+ or CD8+ T-cells.
  It can especially be used to differentiate T-cell lymphomas (CD45RO+) from B cell lymphomas (D45RA+).
  Cellular Localization: Cell membrane.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD163 molecule (CD163), transcript variant 1
• Database Link:
  Entrez Gene 9332 HumanEntrez Gene 5788 Human
• Background: CD45 or Leukocyte Common Antigen (LCA/T200) belongs to a family of membrane sialoglycoproteins with MW of 180kDa-220kDa. Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates various isoforms. CD45RA.is a single chain glycoprotein. It is expressed on appoximately 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. CD45RA are naive or virgin T-cells while, T-cells expressing CD45RO are memory T-cells.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane