CD61 (ITGB3) Mouse Monoclonal Antibody [Clone ID: 2F2]

CAT#: AM10070SU-N

CD61 (ITGB3) mouse monoclonal antibody, clone 2F2, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human bone marrow stained with CD61 antibody Cat.-No. AM10070SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of megakaryocytes.

Specifications

Product Data

• Clone Name: 2F2
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/25-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Bone marrow or tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein encoding part of the external domain of Human CD61
• Specificity: Clone 2f2 will prove useful in detecting neoplastic platelet precursors, normal platelets and most cases of megakaryocytic leukemia.
  Cellular Localization: Cell membrane/cytoplasmic.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens integrin subunit beta 3 (ITGB3)
• Database Link:
  Entrez Gene 3690 Human
• Background: CD61 also known as gpIIIa, the common beta subunit (integrin beta3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, a/b-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or aIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin and vitronectin on activated platelets. With the CD51 antigen (VNR a-chain or av), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin.
  CD61 (GPIIIa) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin.
• Synonyms: Integrin beta-3, GP3A, GPIIIa

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton