SNF5 (SMARCB1) Mouse Monoclonal Antibody [Clone ID: 25]

CAT#: AM10099SU-N

SNF5 (SMARCB1) mouse monoclonal antibody, clone 25, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human astrocytoma stained with INI1 antibody Cat.-No. AM10099SU-N using peroxidase conjugate and DAB chromogen. Note the nuclear staining of malignant glial cells.

Specifications

Product Data

• Clone Name: 25
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Brain or astrocytoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Mouse BAF47 amino acids 257-359
• Specificity: Lack of nuclear labeling by anti-INI-1 is characteristic of MRT. The majority of medulloblastomas and sPNETs are labeled by anti-INI-1 antibody. MRTs also originate from the kidney and soft tissues.
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Concentration: lot specific
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens inhibin alpha subunit (INHA)
• Database Link:
  Entrez Gene 3623 HumanEntrez Gene 6598 Human
• Background: The INI-1 gene, which encodes a functionally uncharacterized protein component of the hSWI/SNF chromatin remodeling complex, is often mutated or deleted in malignant rhabdoid tumor (MRT). Two isoforms of INI-1, that differ by the variable inclusion of amino acids, potentially are produced by differential RNA splicing. The morphology of MRTs can present challenges in differential diagnosis. The overall survival of MRTs relative to its potential mimics (medulloblastoma, supratenorial primitive neuroectodermal tumors (sPNETs)) is quite low, and thus differentiation from these other tumors is desirable.
• Synonyms: Integrase interactor 1 protein, INI1, SNF5 homolog, SNF5L1, BAF47

Reference Data

• Protein Families: Druggable Genome, Secreted Protein