Mammaglobin A (SCGB2A2) Cocktail Monoclonal Antibody [Clone ID: 304-1A5+31-A5]

CAT#: AM10110PU-N

Mammaglobin A (SCGB2A2) cocktail monoclonal antibody, clone 304-1A5+31-A5, Purified

Specifications

Product Data

• Clone Name: 304-1A5+31-A5
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections.
  Recommended Dilution: 1/100-1/500.
  Positive Control: Breast tissue, breast carcinoma.
  Preparation and Pretreatment
  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
• Reactivities: Human
• Host: Cocktail
• Isotype: cocktail
• Clonality: Monoclonal
• Specificity: This Mouse/Rabbit Monoclonal (304-1A5 & 31A5) cocktail antibody Recognizes Mammaglobin (10 kDa).
  When combined with other breast-restricted markers such as GCDFP-15, an overall sensitivity for breast carcinoma of 84% has been achieved.
  Anti-Mammaglobin can also be used to determine breast origin in the setting of metastatic carcinoma.
  Cellular Localization: Cytoplasmic.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction.
  Stabilizer: 0.9% BSA
  Preservative: 0.09% Sodium Azide
• Purification: protein A chromatograpy.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 4250 Human
• Background: Mammaglobin, a member of the uteroglobin family, is a 10kDa glycoprotein. Mammaglobin mRNA expression is breast tissue specific. It is overexpressed in human breast cancer. The use of Mammaglobin RT-PCR has been demonstrated to detect disseminated breast cancer. In a study to evaluate the frequency and specificity of mammaglobin, 80% of all primary and metastatic breast tumors tested were immunopositive irrespective of tumor grade, while mammaglobin mRNA was detected in 60% of the peripheral stem cell collections from breast cancer patients.
• Synonyms: Mammaglobin-A, Mammaglobin-1, Secretoglobin family 2A member 2, MGB1, UGB2, Prostatein peptide C3, Prostatic steroid-binding protein C3
• Note:

  Immunoglobulin type: Mouse IgG1, Rabbit IgG