HMB45 Mouse Monoclonal Antibody [Clone ID: HMB45]

CAT#: AM10112SU-N

HMB45 mouse monoclonal antibody, clone HMB45, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human melanoma stained with HMB45 antibody Cat.-No. AM10112SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of melanoma cells.

Specifications

Product Data

• Clone Name: HMB45
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Melanoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Extract of pigmented melanoma metastases from lymph nodes.
• Specificity: By Immunohistochemistry, HMB-45 specifically recognizes a protein in melanocytes and melanomas.
  Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Background: Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
• Synonyms: HMB45 Melanoma Marker