PAX8 Rabbit Monoclonal Antibody [Clone ID: ZR-1]

CAT#: AM10131SU-N

PAX8 rabbit monoclonal antibody, clone ZR-1, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded endometrial clear cell carcinoma stained with PAX8 antibody Cat.-No. AM10131SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Specifications

Product Data

• Clone Name: ZR-1
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval (EDTA, pH 9.0) is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Ovarian serous carcinoma.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to the C-terminus of Human PAX8 protein.
• Specificity: Recognizes Pax8.
  Cellular Localization: Nuclear.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Stabilizer: 0.2% BSA
  Preservative: 15mM Sodium Azide
• Gene Name: Homo sapiens neurofilament, light polypeptide (NEFL)
• Database Link:
  Entrez Gene 7849 Human
• Background: PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. Studies have also found PAX-8 experession in renal tubules as well as renal carcinoma, nephroblastoma and seminoma. Over 98% of clear cell RCCs, 90% of papillary RCCs, and 95% of oncocytomas were positive for PAX-8, frequencies which are similar or better than for PAX-2. Normal lung and lung carcinomas do not express PAX-8. Similarly, the absence of expression of PAX-8 in breast and other non-GYN carcinomas other than those primary to the thyroid indicates that PAX-8 is an important new marker of ovarian cancer and a useful marker for the differential diagnoses in lung and neck tumors, or tumors at distant sites where primary lung carcinoma or thyroid carcinoma are possibilities. PAX-8, combined with organ system-specific markers such as uroplakin, mammaglobin, and TTF-1 can be a very useful panel to determine the primary site of invasive
  micropapillary carcinomas of ovary from bladder, lung, and breast.
• Synonyms: Pax-8

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)