Collagen V (COL5A1) Mouse Monoclonal Antibody [Clone ID: 13F6]

CAT#: AM10159PU-N

Collagen V (COL5A1) mouse monoclonal antibody, clone 13F6, Purified

Product Images

Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) (Cat.-No AM10159PU-N) Anti-COL5A1 antibody ICC staining of human derma fibroblasts (24h).

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  WB : Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) Cat.-No AM10159PU-N staining of reombinant pNalpha1 homotrimer (1) and pepsinized collagen V heterotrimer (2).

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  Collagen V alpha-1 chain N-propeptide -NC2 domain antibody (13F6) Cat.-No AM10159PU-N staining of human formalin-fixed paraffin-embedded tissue skin after heat induced antigen retrieval.

Specifications

Product Data

• Clone Name: 13F6
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/200-1/1000.
  Immunoprecipitation.
  Immunohistochemistry: 1/100-1/500.
  Immnunocytochemistry: 1/100-1/500.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant N alpha 1 fragment : Human N alpha 1 (V).
  The epitope is located in the COL2-NC2 region.
• Specificity: Reacts with Human pro alpha 1(V) chain.
• Formulation: 0.1M Tris, 0.1M Glycine, 2% Sucrose
  State: Purified
  State: Lyophilized powder
  Preservative: None
• Reconstitution Method: Restore in distilled water
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Lyophilized powder stable for a minimum of 2 years at -20°C.
  Store reconstituted antibody at 2-8°C (in aliquots) at -20°C for longer.

• Stability: Shelf life: Six months from despatch.

• Gene Name: collagen type V alpha 1
• Database Link:
  Entrez Gene 1289 Human
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.
• Synonyms: COL5A1