Digoxigenin Mouse Monoclonal Antibody [Clone ID: 21H8]

CAT#: AM10164HR-N

Digoxigenin mouse monoclonal antibody, clone 21H8, HRP

Specifications

Product Data

• Clone Name: 21H8
• Applications: ELISA, IHC, ISH, SB, WB
• Recommended Dilution:

  ELISA: 1/1000-1/10000.
  Southern Blot: 1/500-1/5000.
  Western Blot: 1/500-1/5000.
  In Situ Hybridization: 1/200-1/2000.
  Immunohistochemistry on Frozen Sections: 1/200-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/2000.

• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Digoxigenin
• Specificity: Reacts with free and bound Digoxigenin.
• Formulation: PBS, pH 7.4
  Label: HRP
  State: Lyohilized purified IgG fraction
  Stabilizer: 50% Glycerol, 1% BSA
  Preservative: 0.02% Thiomersal
• Purification: Affinity Chromatography on Protein A
• Conjugation: HRP
• Background:

  Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.