Digoxigenin Mouse Monoclonal Antibody [Clone ID: 21H8]
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Specifications
Product Data | |
Clone Name | 21H8 |
Applications | ELISA, IHC, ISH, WB |
Recommended Dilution | ELISA: 1/1000-1/10000. Southern Blot: 1/500-1/5000. Western Blot: > 1/500. In Situ Hybridization. Immunohistochemistry: 1/200-1/2000. |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Digoxigenin |
Specificity | Reacts with free and bound Digoxigenin. |
Formulation | 0.1M Tris, 0.1M Glycine, 2% Sucrose State: Purified State: Lyophilized powder Preservative: None |
Reconstitution Method | Restore in distilled water |
Concentration | 1.0 mg/ml |
Purification | Protein A Chromatography |
Background | Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. |
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