GFP Mouse Monoclonal Antibody [Clone ID: 168AT1211]

CAT#: AM11009PU-N

GFP mouse monoclonal antibody, clone 168AT1211, Purified

Product Images

Top: Western blot analysis of Monoclonal anti-GFP antibody (Cat.-No AM11009PU-N) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab. But

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  Formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag Antibody (Cat.-No AM11009PU-N ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -ve shows negative staining of the antibody in

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  Immuno-fluorescence for GFP Tag Antibody (Cat.-No AM11009PU-N). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing c

Specifications

Product Data

• Clone Name: 168AT1211
• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting: 1/4,000.
  Immunohistochemistry: 1/50-1/100.
  Immunofluorescence: 1/50-1/100.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified His-tagged GFP protein was used to produced this monoclonal antibody.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 27 kDa
• Database Link:
  UniProt ID P42212
• Background: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
• Synonyms: Green fluorescent protein, GFP-Tag