CD162 (SELPLG) Mouse Monoclonal Antibody [Clone ID: TC2]

CAT#: AM12002RP-N

CD162 (SELPLG) mouse monoclonal antibody, clone TC2, PE

Product Images

Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2).

Specifications

Product Data

• Clone Name: TC2
• Applications: FC
• Recommended Dilution: Flow Cytometry: 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human thymocytes
• Specificity: This antibody reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34⁺ bone marrow cells.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free BSA
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin under optimum conditions.
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens selectin P ligand (SELPLG), transcript variant 2
• Database Link:
  Entrez Gene 6404 Human
• Background: CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.
• Synonyms: PSGL-1, Selectin P ligand, SELPLG

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)