ENPP3 Mouse Monoclonal Antibody [Clone ID: NP4D6]

CAT#: AM12003PE-N

ENPP3 mouse monoclonal antibody, clone NP4D6, PE

Product Images

Surface staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).

• 

  Surface staining of human basophils in allergen-stimulated whole blood (CD203cpos/ SSClow) by anti-CD203c antibody (NP4D6).

Specifications

Product Data

• Clone Name: NP4D6
• Applications: FC, IF
• Recommended Dilution: Flow Cytometry analysis of Human blood cells using 20 µl reagent/100 ml of whole blood or 10e6 cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
  Immunofluorescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HEK-293 cells transfected with Human CD203c
• Specificity: The antibody NP4D6 reacts with CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
• Formulation: PBS
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% high-grade protease free BSA as a stabilizing agent
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!.
  This antibody is photosensitive and should be protected from light.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), transcript variant 1
• Database Link:
  Entrez Gene 5169 Human
• Background: CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
• Synonyms: E-NPP 3, PDNP3, Phosphodiesterase I beta, PD-I beta

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Pantothenate and CoA biosynthesis, Purine metabolism, Riboflavin metabolism, Starch and sucrose metabolism