Cd247 Hamster Monoclonal Antibody [Clone ID: H146-968]

CAT#: AM12004BT-N

Cd247 hamster monoclonal antibody, clone H146-968, Purified

Specifications

Product Data

• Clone Name: H146-968
• Applications: FC, IF
• Recommended Dilution: This clone (H146-968) has been reported to work in Flow Cytometry (in permeabilized cells), Western Blotting and ELISA.
• Reactivities: Human, Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide KLH conjugated corresponding to amino acids 151-164 of Mouse CD3 zeta
• Specificity: This antibody detects the zeta chain subunit of the T cell receptor (TCR)(CD247).
• Formulation: PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
  State: Purified
  State: Liquid purified Ig fraction.
  Label: Biotin
• Concentration: 0.1 mg/ml
• Purification: Protein G Affinity Chromatography.
• Gene Name: Mus musculus CD247 antigen (Cd247), transcript variant zeta
• Database Link:
  Entrez Gene 919 HumanEntrez Gene 12503 Mouse
• Background: The TCR complex consists of a ligand specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma, delta, eta and zeta subunits. This complex regulates assembly and expression of the receptor and is thought to be responsible for transmembrane transduction of signals after binding to TCRαβ.
  Studies have suggested that the CD3z subunit (also know as CD247) plays an important role in two distinct cellular compartments. In the cytoplasm, it may function to regulate Ag receptor expression and in the plasma membrane it may be required for optimal signaling by the physiologic ligand Ag/MHC. The CD3z subunit is also associated with the CD16 IgG Fc receptor on NK cells.
• Synonyms: T3Z, TCRZ, CD3 zeta chain