Dlg4 Mouse Monoclonal Antibody [Clone ID: 6G6]

CAT#: AM12013PU-S

Dlg4 mouse monoclonal antibody, clone 6G6, Purified

Specifications

Product Data

• Clone Name: 6G6
• Applications: IF, IHC, WB
• Recommended Dilution: Western blot.
  Immunocytochemistry.
  Immunflourescence
  Immunohistochemistry on frozen sections.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant rat PSD-95
• Specificity: This antibody detects an ~100 kDa protein corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE immunoblots. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.
• Formulation: PBS pH 7.4, in 0.09% azide in 50% glycerol
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G chromatography
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Rattus norvegicus discs large MAGUK scaffold protein 4 (Dlg4)
• Database Link:
  Entrez Gene 1742 HumanEntrez Gene 13385 MouseEntrez Gene 29495 Rat
• Background: Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an Nterminal variable segment followed by three aminoterminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4).
  PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).
• Synonyms: DLGH4, PSD-95, Disks large homolog 4, SAP90, SAP-90