IRS1 pSer616 Mouse Monoclonal Antibody [Clone ID: HIR-B1]

CAT#: AM12122PU-N

IRS1 pSer616 mouse monoclonal antibody, clone HIR-B1, Aff - Purified

Product Images

Indirect ELISA: Serial dilution of the antibody starting with 5 ug/well was done to check for the affinity. 1 ug of coated antigen can be sensitively detected by anti-Human IRS pSer616 antibody used at 300 pg/well concentration.

Specifications

Product Data

• Clone Name: HIR-B1
• Applications: ELISA, WB
• Recommended Dilution: Indirect ELISA: Use at 2 µg/ml (For detection of antigen).
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide phosphorylated at Ser616 site.
• Specificity: This antibody does not recognize IRS peptide Not phosphorylated at Ser616 and will not recognize IRS peptide phosphorylated at Tyr183.
  Also does not react with Riboflavin carrier protein which is known to have multiple phosphate groups.
• Formulation: PBS containing containing 30% Glycerol, 0.5% BSA and 0.01% Thimerosal.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Storage: Store the antibody (in aliquots) at -20°C
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens insulin receptor substrate 1 (IRS1)
• Database Link:
  Entrez Gene 3667 Human
• Background: Insulin receptor substrates (IRS) subtypes are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. The balance between Ser/Thr phosphorylation of IRS has been demonstrated to be an important regulator of insulin signaling. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas.
• Synonyms: Insulin receptor substrate 1, IRS-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus