Cd3e Hamster Monoclonal Antibody [Clone ID: 145-2C11]

CAT#: AM12132LE-N

Cd3e hamster monoclonal antibody, clone 145-2C11, Low Endotoxin

Product Images

Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.

Specifications

Product Data

• Clone Name: 145-2C11
• Applications: FC, FN, IF, IHC, IP
• Recommended Dilution: Flow Cytometry: 1-2 μg / ml (million cells).
  Immunoprecipitation: 1-2 μg / 100-500 μg protein in 1 ml cell lysate.
  Immunohistochemistry (frozen sections).
  Immunocytochemistry.
  Functional Application: Induction of T cell activation, proliferation or apoptosis (depending on conditions); in vivo T cell depletion.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Mouse BM10-37 cytotoxic T lymphocytes
• Specificity: This antibody reacts with mouse CD3 (epsilon subunit). It is commonly used as a phenotypic marker for mouse T cells.
• Formulation: Phosphate buffered saline (PBS), approx. pH 7.4
  State: Low Endotoxin
  State: Liquid Ig fraction
• Concentration: 1.0 mg/ml (> 95% pure by SDS-PAGE)
• Purification: Protein A
• Gene Name: Mus musculus CD3 antigen, epsilon polypeptide (Cd3e)
• Database Link:
  Entrez Gene 12501 Mouse
• Background: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
• Synonyms: T3/Leu-4