Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: 4C2]

CAT#: AM12139RP-N

Human Lambda Light Chain mouse monoclonal antibody, clone 4C2, PE

Product Images

B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.

Specifications

Product Data

• Clone Name: 4C2
• Applications: FC
• Recommended Dilution: Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
  Label: PE
  State: Liquid purified Ig fraction
  Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use
• Conjugation: PE
• Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.