CD229 (LY9) Mouse Monoclonal Antibody [Clone ID: HLy9.25]

CAT#: AM12140LE-N

CD229 (LY9) mouse monoclonal antibody, clone HLy9.25, Low Endotoxin

Specifications

Product Data

• Clone Name: HLy9.25
• Applications: FC, FN, IF, IP, WB
• Recommended Dilution: Flow cytometry: 6 μg/ml. Positive control: peripheral blood lymphocytes.
  Immunoprecipitation.
  Western blotting.
  Immunocytochemistry.
  Functional application: Regulation of activation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD299-transfected 300.19 pre-B cell line
• Specificity: The antibody recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Low Endotoxin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens lymphocyte antigen 9 (LY9), transcript variant 1
• Database Link:
  Entrez Gene 4063 Human
• Background: CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
• Synonyms: Ly-9

Reference Data

• Protein Families: Transmembrane