Integrin alpha-7 / ITGA7 Mouse Monoclonal Antibody [Clone ID: 3C12]

CAT#: AM20011RP-N

Integrin alpha-7 / ITGA7 mouse monoclonal antibody, clone 3C12, PE

Product Images

Flow Cytometric analysis of mouse Integrin alpha-7 expression on NIH/3T3 (Left) and C2C12 (Right). Open Histogram indicates the reaction of Isotypic Control to the cells. Shaded Histograms indicate the reaction of ITGA7 antibody to the cells.

Specifications

Product Data

• Clone Name: 3C12
• Applications: FC
• Recommended Dilution: Flow Cytometry: 20 µl (Ready for Use).
  Positive Control: C2C12.
  Detailed Procedure is provided in the following Protocols.
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mouse myoblasts.
• Specificity: This antibody reacts with Mouse Integrin alpha-7 on Flow Cytometry.
• Formulation: PBS containing 1% BSA as stabilizer and 0.09% Sodium Azide as preservative.
  Label: PE
  State: Liquid purified IgG fraction.
• Purification: Protein-A Agarose Chromatography.
• Conjugation: PE
• Background: The integrin family of adhesion molecules participate in important cell-cell and cell-extracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of an alpha subunit and a beta subunit. Both alpha and beta subunits are transmembrane proteins with large extracellular domains (>100 kDa for alpha subunit and >75 kDa for beta subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the beta-4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed “inside-out” signaling. It has been reported that, upon ligand binding, integrins regulate many intracellular signaling pathways that involve cytoplasmic alkalization, intracellular Ca2+ fluctuation, inositol lipid metabolism, protein kinase C, MAP kinase and phosphatidyl inositol kinase. Integrin alpha-7 is a specific cellular receptor for the basement membrane protein laminin-1, as well as for the laminin isoforms-2 and -4. The alpha-7 subunit is expressed mainly in skeletal and cardiac muscle and may be involved in differentiation and migration processes during myogenesis. Absence of integrin alpha-7 results in muscular dystrophy is revealed.
• Note: This product was originally produced by MBL International.
  
  Protocol: Flow Cytometric Analysis for Floating Cells
  We usually use Fisher tubes or equivalents as reaction tubes for all step described below.
  1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and 0.1% NaN3].
  2) Resuspend the cells with washing buffer (5x10e6 cells/mL).
  3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25°C). Remove supernatant by careful aspiration.
  4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0.1% NaN3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature.
  5) Add 20 µL the PE labeled anti-mouse Integrin alpha-7 monoclonal antibody (3C12). Mix well and incubate for 30 minutes at room temperature.
  6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration.
  7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
  Positive Control for Flow Cytometry: C2C12