CD30 (TNFRSF8) Mouse Monoclonal Antibody [Clone ID: Ber-H2]

CAT#: AM20190SU-N

CD30 (TNFRSF8) mouse monoclonal antibody, clone Ber-H2, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human Hodgkin's lymphoma stained with CD30 antibody Cat.-No. AM20190SU-N using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of Hodgkin's cells.

Specifications

Product Data

• Clone Name: Ber-H2
• Applications: IHC, WB
• Recommended Dilution: Western Blot.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Hodgkin's lymphoma or anaplastic large cell lymphoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Co cell line established from a patient with Hidgkin's disease of T-cell lineage.
• Specificity: This antibody reacts with CD30 antigen.
  Ber-H2 distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
  Cellular Localization: Cell membrane and cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid purified Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens CD163 molecule (CD163), transcript variant 1
• Database Link:
  Entrez Gene 9332 HumanEntrez Gene 943 Human
• Background: CD30, a single chain glycoprotein, is synthesized as a 90kDa precursor which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. The CD30/Ki-1 antigen is expressed by mononuclear Hodgkin and multinucleated Reed-Sternberg cells in Hodgkin’s disease, by the tumor cells of a majority of anaplastic large cell lymphomas, and by a varying proportion of activated T and B cells. B. Ber-H2 distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
• Synonyms: TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane