ATG5 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C3]

CAT#: AM20206PU-N

ATG5 (incl. pos. control) mouse monoclonal antibody, clone 11C3, Purified

Product Images

Whole lysate of serum starved SH-SY5Y cells was applied to SDS-PAGE and tranferred to a PVDF membrane. The Immunoblot was probed with the indicated monoclonal antibodies at 0.5 ug/ml for 1h at 15-22°C and developed with ECL (exposure time: 30 sec). Lane M: Molecular Weight marker Lane 1: AM20205PU-N (Clone 7C6) Lane 2: AM20206PU-N (Clone 11C3)

Specifications

Product Data

• Clone Name: 11C3
• Applications: WB
• Recommended Dilution: Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer.
  Included Postitive Control: Cell lysate from untreated SH-SY5Y cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant ATG5
• Specificity: Recognizes the ATG5-ATG12 protein complex at 55 kDa in immunoblot application.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: PEG and Sucrose
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1ml H₂O (15 min, RT).
• Purification: Purified from Serum-Free Cell Culture Supernatant by Subsequent Ultrafiltration and Size Exclusion Chromatography
• Gene Name: Homo sapiens autophagy related 5 (ATG5), transcript variant 1
• Database Link:
  Entrez Gene 11793 MouseEntrez Gene 365601 RatEntrez Gene 9474 Human
• Background: Autophagy as a response to cellular stress and starvation is an alternative process of proteasomal degradation for some long-lived proteins and organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). The gene product of autophagy-related gene 5 (ATG5) is required for autophagosome-formation. ATG5 also enhances the susceptibility towards apoptotic stimuli. Like Bcl-2, ATG5 exhibits a dual function by modulating both autophagy and apoptosis.
• Synonyms: ASP, APG5-like, Autophagy protein 5
• Note: Molecular Weight: 24, 33, 55 kDa
  
  Protocol: Positive Control: Cell lysate from untreated SH-SY5Y cells.
  Formulation: Lyophilized cell lysate from Serum starved SH-SY5Y cells.
  Stability:
  Reconstitute by addition of 200 µl H₂O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  Application:
  The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells.
  Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Storage:
  Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  Shelf life: one year from despatch.
  The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy, RIG-I-like receptor signaling pathway