Brk (PTK6) Mouse Monoclonal Antibody [Clone ID: 24B7]

CAT#: AM20211PU-N

Brk (PTK6) mouse monoclonal antibody, clone 24B7, Purified

Specifications

Product Data

• Clone Name: 24B7
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols for more details).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin.
  Epitope: Internal peptide derived from BRK/PTK6 sequence
• Specificity: Recognizes BRK/PTK6
• Formulation: PBS containing 0.09% Sodium Azide, PEG, Sucrose and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Storage: Store the antibody in aliquots) at -20°C.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens protein tyrosine kinase 6 (PTK6), transcript variant 1
• Database Link:
  Entrez Gene 5753 Human
• Background: This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.
• Synonyms: Tyrosine-protein kinase 6, Breast tumor kinase
• Note: Molecular Weight: 52 kDa
  
  Protocol: Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma (Human)
  
  Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
  
  Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein