Her2 (ERBB2) pTyr877 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12B9]

CAT#: AM20215PU-N

Her2 (ERBB2) pTyr877 (incl. pos. control) mouse monoclonal antibody, clone 12B9, Purified

Specifications

Product Data

• Clone Name: 12B9
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Included Positive Control: Cell lysate from EGF-treated SKOV-3 cells (See Protocols for more details).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopetide conjugated to hemocyanin
  Epitope: Phospho-Tyr877
• Specificity: Recognizes ErbB2 phosphorylated at Tyrosine 877 at 185 kDa.
• Formulation: PBS, 0.09% Sodium Azide/PEG and Sucrose.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Thiophilic and Size Exclusion Chromatography.
• Storage: Stzore the antibody (in aliquots) -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 2
• Database Link:
  Entrez Gene 2064 Human
• Background: ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
• Note: Mol. weight: 185 kDa.
  
  Protocol: Positive Control Provided.
  Cell lysate from EGF-treated SKOV-3 cells
  
  Description: Cell lysate from EGF-treated SKOV-3 cells, ovary adenocarcinoma (Human).
  
  Format: Lyophilized cell lysate from SKOV-3 cells.
  Serum starved cells were treated for 15min with EGF.
  
  Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer