PAK4 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C1]

CAT#: AM20217PU-N

PAK4 (incl. pos. control) mouse monoclonal antibody, clone 6C1, Purified


USD 465.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name 6C1
Applications WB
Recommended Dilution Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for more details).
Reactivities Canine, Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Peptide conjugated to hemocyanin
Epitope: Peptide derived from the kinase activation loop.
Specificity Recognizes p21 activated kinase 4 (PAK4).
Formulation 1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose.
State: Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore with 1 ml H2O (15 min, RT).
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Gene Name Homo sapiens p21 (RAC1) activated kinase 4 (PAK4), transcript variant 2
Background p21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.
Synonyms PAK 4, PAK-4, p21-activated kinase 4, KIAA1142
Note Mol. weight: 60 kDa

Protocol: Positive Control Provided.
Cell lysate from untreated SW480 cells


Description: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)

Format: Lyophilized cell lysate from serum starved SW480 cells.

Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Storage: Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway

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