Her2 (ERBB2) pTyr1139 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14C3]

CAT#: AM20224PU-N

Her2 (ERBB2) pTyr1139 (incl. pos. control) mouse monoclonal antibody, clone 14C3, Purified

Specifications

Product Data

• Clone Name: 14C3
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Included Positive Control: Cell lysate from EGF-treated SKOV-3 cells (See Protocols for more details).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Phosphopetide conjugated to hemocyanin
  Epitope: Phospho-Tyr1139
• Specificity: This 14C3 antibody specifically recognizes erbB2 phosphorylated at tyrosine 1139 at 185 kDa.
• Formulation: 1ml PBS, containing 0.09% Sodium Azide/PEG and sucrose
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Storage: For long-term storage, freeze lyophilizate upon arrival (-20°C).
  Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year.
  Avoid repeated freeze / thaw cycles.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
• Gene Name: Homo sapiens erb-b2 receptor tyrosine kinase 2 (ERBB2), transcript variant 2
• Database Link:
  Entrez Gene 2064 Human
• Background: erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.
• Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19
• Note: Mol. weight: 185 kDa
  
  Protocol: Positive Control Provided.
  Cell lysate from EGF-treated SKOV-3 cells
  
  Description: Cell lysate from EGF-treated SKOV-3 cells, ovary adenocarcinoma (Human)
  
  Format: Lyophilized cell lysate from serum starved EGF-treated SKOV-3 cells.
  
  Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer