EGFR (Extracell. non Ligand binding Site) Mouse Monoclonal Antibody [Clone ID: EGF-R2]

CAT#: AM20237PU-N

EGFR (Extracell. non Ligand binding Site) mouse monoclonal antibody, clone EGF-R2, Purified


USD 340.00

2 Weeks*

Size
    • 100 ug

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Other products for "EGFR"

Specifications

Product Data
Clone Name EGF-R2
Applications Assay, ELISA, FC, IHC
Recommended Dilution ELISA (Ref.1-3).
Flow Cytometry.
Immunocytochemistry (Ref.2).
Immunohistochemistry on Cryosections.
Immunohistochemistry on Paraffin Sections. 
Antigen Retrieval:
Heat the deparaffinized, rehydrated sections 2x in fresh citrate buffer pH 6.0 for 5 min. in microwave.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human EGF-Receptor
Specificity Recognizes Human EGF-Receptor (extracellular non ligand binding site).
There was no cross reactivity obtained with v-erb-B
Formulation PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
State: Purified
State: Lyophilized purifed IgG fraction from Cell Culture Supernatant
Reconstitution Method Restore in aqua bidest to 1 mg/ml
Purification Protein G Chromatography
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Synonyms Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.