EGFR (Extracell. non Ligand binding Site) Mouse Monoclonal Antibody [Clone ID: EGF-R2]

CAT#: AM20237PU-N

EGFR (Extracell. non Ligand binding Site) mouse monoclonal antibody, clone EGF-R2, Purified

Product Images

Figure 1. Electron Microscopy Image of internalized EGF-R antibody complexes in A431 cells. The cells were stained with AM20237PU-N/AM20237PU-S for 1h at 4°C, followd by Peroxidase-conjugated Goat anti-Mouse IgG. After fixation with 2.5% glutaraldehyde, the signal was detected using DAB (0.6 mg/ml) for 20 min at RT. The cells were then processed for the preparation of ultrathin sections. Reins HA at al. (1993) J. Cell. Biochem 51 (2): 236-48.

Specifications

Product Data

• Clone Name: EGF-R2
• Applications: Assay, ELISA, FC, IHC
• Recommended Dilution: ELISA (Ref.1-3).
  Flow Cytometry.
  Immunocytochemistry (Ref.2).
  Immunohistochemistry on Cryosections.
  Immunohistochemistry on Paraffin Sections.
  Antigen Retrieval: Heat the deparaffinized, rehydrated sections 2x in fresh citrate buffer pH 6.0 for 5 min. in microwave.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human EGF-Receptor
• Specificity: Recognizes Human EGF-Receptor (extracellular non ligand binding site).
  There was no cross reactivity obtained with v-erb-B
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Lyophilized purifed IgG fraction from Cell Culture Supernatant
• Reconstitution Method: Restore in aqua bidest to 1 mg/ml
• Purification: Protein G Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
• Database Link:
  Entrez Gene 1956 Human
• Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton