Dityrosine / DT Mouse Monoclonal Antibody [Clone ID: 1C3]

CAT#: AM20243PU-S

Dityrosine / DT mouse monoclonal antibody, clone 1C3, Purified

Product Images

Figure 2. Western blot analysis od Dityrosine -cross-linked Human Amyloid Beta. 200 ng of Abeta 1-40 peptides before and after Cu (II)-Oxidation were separated by SDS-PAGE and immunblotted with AM20243PU-S. AM20243PU-S detects dimeric and trimeric Abeta 1-4

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  Figure 1. Immunohistochemistry image of Dityrosine staining in Paraffin Section of Human atherosclerotic lesion. The section was activated in Vector Antigen Unmasking Solution at 121°C for 10min prior to blocking. The section was incubated with AM20243PU-S

Specifications

Product Data

• Clone Name: 1C3
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA (Ref.1,2)
  Western blot: 4 µg/ml (Ref.3,5)
  Immunohistochemistry on Paraffin Sections (Ref.4,6).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: 3-(p-hydroxyphenyl) Propionic Acid Dimer, KLH-conjugated
• Specificity: Specific for free Dityrosine, 3-(p-hydroxyphenyl) Propionic Acid dimer, Dityrosine-BSA conjugate, and Dityrosine in proteins or peptides.
  There was no cross reactivity detected for following compounds: Tyrosine, Nitrotyrosine, Chlorotyrosine, Aminotyrosine, Phosphotyrosine, 3-(phydroxyphenyl) Propionic Acid, Phenylalanine, 3,4-Dihydroxyphenylacetic Acid (DOPA).
• Formulation: 10mM PBS, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 0.2 ml aqua bidest to 0.1 mg/ml of IgG
• Concentration: 0.1 mg/ml (after reconstitution)
• Purification: Protein A Chromatography
• Background: Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains.
  Dityrosine is one of the specific biomarkers for protein oxidation.