Human Kappa Light Chain (free and bound) Mouse Monoclonal Antibody [Clone ID: NI 285, NI 250]

CAT#: AM20244BT-N

Human Kappa Light Chain (free and bound) mouse monoclonal antibody, clone NI 285, NI 250, Biotin

Specifications

Product Data

• Clone Name: NI 285, NI 250
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, Indirect Immunoperoxidase staining and immunoblotting.
  General Recommended Dilutions:
  Histochemical Use: 1/100-1/500
  ELISA: from 1/1000 upwards
  Western blot: from 1/2000 upwards.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified Bence Jones kappa proteins isolated from pooled Human urine
• Specificity: The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
  The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding Enzyme Immunoassay, Imunoblotting, Immunoprecipitation and Direct Immunoperoxidase staining.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Label: Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life
  Molar radio: Biotin/IgG: ~2.0
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 0.4 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin