Human Kappa Light Chain (free and bound) Mouse Monoclonal Antibody [Clone ID: NI 285, NI 250]

CAT#: AM20244TC-N

Human Kappa Light Chain (free and bound) mouse monoclonal antibody, clone NI 285, NI 250, TRITC


USD 410.00

2 Weeks*

Size
    • 200 ug

Product Images

Specifications

Product Data
Clone Name NI 285, NI 250
Applications ELISA, IF, IHC, IP, WB
Recommended Dilution To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, Indirect Immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/20-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1000 upwards.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Highly purified Bence Jones kappa proteins isolated from pooled Human urine
Specificity The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
The reactivity of this preparation of two monoclonal antibodies is restricted to a surface determinant on the kappa light chain. It is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding Enzyme Immunoassay, Imunoblotting, Immunoprecipitation and Direct Immunoperoxidase staining.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: TRITC
State: Lyophilized purified IgG fraction
Label: Tetramethylrhodamine Isothiocyanate
Absorption emission: 554 nm / 573 nm
Molar radio: TRITC/IgG: ~4.5
Reconstitution Method Restore by adding 0.5 ml sterile distilled water.
Concentration 0.4 mg/ml
Purification Immunoaffinity Chromatography
Conjugation TRITC
Note Fluorescence marker: Tetramethylrhodamine isothiocyanate isomer R with an orange-red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching.
Conjugation Procedure: A proprietary technique for the binding to TRITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.