TRITC Mouse Monoclonal Antibody [Clone ID: NI 308]

CAT#: AM20264HR-N

TRITC mouse monoclonal antibody, clone NI 308, HRP

Specifications

Product Data

• Clone Name: NI 308
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/40
  ELISA: from 1/100 upwards.
  Western blot: from 1/200 upwards.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified Tetramethylrhodamine Isothiocyanate isomer R.
• Specificity: The reactivity of the antiserum is directed to TRITC as tested in direct binding enzyme immunoassay, ELISA and Immunoblotting.
  This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
• Formulation: PBS, pH 7.2 without foreign proteins.
  Label: HRP
  State: Lyophilized purified IgG fraction.
  Label: Horseradish Peroxidase
  Molar radio: Peroxidase/IgG ~1.7
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
• Concentration: 0.4 mg/ml
• Conjugation: HRP
• Synonyms: Tetramethylrhodamine Isothiocyanate Isomer 1