SMAD3 Mouse Monoclonal Antibody [Clone ID: AF9F7]

CAT#: AM20332PU-N

SMAD3 mouse monoclonal antibody, clone AF9F7, Purified

Product Images

AM20332PU-N SMAD3 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Kidney at 1/50 followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Clone Name: AF9F7
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 1/50.
  Immunoprecipitation.
  Western Blot: 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human protein purified from E. coli (His-Smad3)
• Specificity: This antibody detects SMAD3.
• Formulation: HEPES with 0.15M NaCl, 0.01% BSA, 0.03% Sodium Azide and 50% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens SMAD family member 3 (SMAD3), transcript variant 1
• Database Link:
  Entrez Gene 17127 MouseEntrez Gene 25631 RatEntrez Gene 4088 Human
• Background: Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
• Synonyms: SMAD family member 3, SMAD 3, SMAD-3, Mad3, MAD-3, MADH3, MAD homolog 3, JV15-2

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway