Flt4 Rat Monoclonal Antibody [Clone ID: 7A3]

CAT#: AM20378PU-N

Flt4 rat monoclonal antibody, clone 7A3, Purified

Product Images

Western analysis with anti-Mouse VEGFR-3/FLT-4 antibody DM3505P at 5 ug/ml (Lysate of 2x10e6 cells).

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  Immunofluorescence staining of VEGFR-3/FLT4 in Mouse primary endothelial cells (SnoMec) with monoclonal Rat anti-Mouse VEGFR-3/FLT4 #7A3 (upper panel). Control with goat anti-rat ALEXA Flour 488 (lower panel).

Specifications

Product Data

• Clone Name: 7A3
• Applications: IF, WB
• Recommended Dilution: Western Blot: 1-5 µg/ml.
  Immunofluorescence: 2-10 µg/ml.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified, insect cell-derived recombinant Mouse soluble FLT-4 (120 kDa).
• Specificity: This antibody will detect Mouse VEGFR-3/FLT-4 in Western Blot in PAEC/FLT-4 cells.
• Formulation: PBS pH 7.4 without preservatives
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: None
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Protein G Chromatography
• Gene Name: Mus musculus FMS-like tyrosine kinase 4 (Flt4)
• Database Link:
  Entrez Gene 14257 Mouse
• Background: Mature native mouse VEGF Receptor 3 (Flt4) is composed of a 751 amino acid residue extracellular domain, a 22 amino acid residue transmembrane domain, and a 565 amino acid residue cytoplasmic domain. Recombinant mature mouse VEGF Receptor 3/Fc is a disulfidelinked dimeric protein. Based on N-terminal sequencing, recombinant mouse VEGF Receptor 3/Fc has Met 17 at the amino-terminus. The amino acid sequence of mouse VEGF Receptor 3 is 88 % identical to human VEGF Receptor 3. VEGF Receptor 3 is a specific marker for lymphatic vessels. In adults, VEGF Receptor 3 expression is restricted to endothelial cells of the lymphatic vessels. It has also been detected on some high endothelial venules, in embryonic prelymphatic blood vessels, in some tumor blood vessels, and in certain hematopoietic and leukemia cells. The predominant role of VEGF Receptor 3 is its involvement in the development of the lymphatic vessel system. Both VEGFC and VEGFD bind and activate the receptors, VEGF Receptor 3 and VEGF Receptor 2.
• Synonyms: VEGFR3, FLT4, VEGF Receptor 3